Monday, January 4, 2010

inGAP: an integrated next-generation genome analysis pipeline

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Summary: We develop a novel mining pipeline, Integrative Next-generation Genome Analysis Pipeline (inGAP), guided by a Bayesian principle to detect single nucleotide polymorphisms (SNPs), insertion/deletions (indels) by comparing high-throughput pyrosequencing reads with a reference genome of related organisms. inGAP can be applied to the mapping of both Roche/454 and Illumina reads with no restriction of read length. Experiments on simulated and experimental data show that this pipeline can achieve overall 97% accuracy in SNP detection and 94% in the finding of indels. All the detected SNPs/indels can be further evaluated by a graphical editor in our pipeline. inGAP also provides functions of multiple genomes comparison and assistance of bacterial genome assembly.

Availability: inGAP is available at http://sites.google.com/site/nextgengenomics/ingap

Contact: scs@bx.psu.edu

Supplementary information: Supplementary data are available at Bioinformatics online.


(source URL, Via Bioinformatics - current issue.)