Friday, July 2, 2010

Manipulation of FASTQ data with Galaxy

Summary: Here, we describe a tool suite that functions on all of the commonly known FASTQ format variants and provides a pipeline for manipulating next generation sequencing data taken from a sequencing machine all the way through the quality filtering steps.

Availability and Implementation: This open-source toolset was implemented in Python and has been integrated into the online data analysis platform Galaxy (public web access:; download: Two short movies that highlight the functionality of tools described in this manuscript as well as results from testing components of this tool suite against a set of previously published files are available at


Supplementary information: Supplementary data are available at Bioinformatics online.
(this Post content was reproduced from:, Via Bioinformatics - current issue.)