Thursday, August 4, 2011

Methyl-Analyzer--whole genome DNA methylation profiling

Methyl-Analyzer--whole genome DNA methylation profiling:

Summary: Methyl-Analyzer is a python package that analyzes genome-wide DNA methylation data produced by the Methyl-MAPS (methylation mapping analysis by paired-end sequencing) method. Methyl-MAPS is an enzymatic-based method that uses both methylation-sensitive and -dependent enzymes covering >80% of CpG dinucleotides within mammalian genomes. It combines enzymatic-based approaches with high-throughput next-generation sequencing technology to provide whole genome DNA methylation profiles. Methyl-Analyzer processes and integrates sequencing reads from methylated and unmethylated compartments and estimates CpG methylation probabilities at single base resolution.

Availability and implementation: Methyl-Analyzer is available at http://github.com/epigenomics/methylmaps. Sample dataset is available for download at http://epigenomicspub.columbia.edu/methylanalyzer_data.html.

Contact:fgh3@columbia.edu

Supplementary information:Supplementary data are available at Bioinformatics online.


(Via Bioinformatics - current issue.)