Saturday, March 3, 2012

Fwd: Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks

Fwd: please follow footer link
Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks:

Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks

Nature Protocols 7, 562 (2012).
doi:10.1038/nprot.2012.016

Authors: Cole Trapnell, Adam Roberts, Loyal Goff, Geo Pertea, Daehwan Kim, David R Kelley, Harold Pimentel, Steven L Salzberg, John L Rinn & Lior Pachter

Recent advances in high-throughput cDNA sequencing (RNA-seq) can reveal new genes and splice variants and quantify expression genome-wide in a single assay. The volume and complexity of data from RNA-seq experiments necessitate scalable, fast and mathematically principled analysis software. TopHat and Cufflinks are free, open-source



(Original Post: Nature Protocols.)