Saturday, March 16, 2013

Fwd: RNA-Seq: revelation of the messengers.

Fwd: please follow footer link

RNA-Seq: revelation of the messengers.: "Publication Date: 2013 Mar 4 PMID: 23481128
Authors: Van Verk, M. C. - Hickman, R. - Pieterse, C. M. - Van Wees, S. C.
Journal: Trends Plant Sci

Next-generation RNA-sequencing (RNA-Seq) is rapidly outcompeting microarrays as the technology of choice for whole-transcriptome studies. However, the bioinformatics skills required for RNA-Seq data analysis often pose a significant hurdle for many biologists. Here, we put forward the concepts and considerations that are critical for RNA-Seq data analysis and provide a generic tutorial with example data that outlines the whole pipeline from next-generation sequencing output to quantification of differential gene expression.

(Via Trends in plant science.)