Sunday, April 7, 2013

Fwd: Genome sequencing of bacteria: sequencing, 'de novo' assembly and rapid analysis using open source tools

Fwd: please follow footer link

Genome sequencing of bacteria: sequencing, 'de novo' assembly and rapid analysis using open source tools: "Veljo Kisand, Teresa Lettieri
BMC Genomics 2013, 14:211 (1 April 2013)"



Background
De novo genome sequencing of previously uncharacterized microorganisms has the potential to open up new frontiers in microbial genomics by providing insight into both functional capabilities and biodiversity. Until recently, Roche 454 pyrosequencing was the NGS method of choice for de novo assembly because it generates hundreds of thousands of long reads (<450 bps), which are presumed to aid in the analysis of uncharacterized genomes. The array of tools for processing NGS data are increasingly free and open source and are often adopted for both their high quality and role in promoting academic freedom.

(Via BMC Genomics most viewed.)