Friday, May 10, 2013

Fwd: FACS purification of Drosophila larval neuroblasts for next-generation sequencing

Choose the input rather than dig in the output, clean approach!
Fwd: please follow footer link

FACS purification of Drosophila larval neuroblasts for next-generation sequencing: "

Nature Protocols 8, 1088 (2013).
doi:10.1038/nprot.2013.062

Authors: Heike Harzer, Christian Berger, Ryan Conder, Gerald Schmauss & Juergen A Knoblich

Elegant tools are available for the genetic analysis of neural stem cell lineages in Drosophila, but a methodology for purifying stem cells and their differentiated progeny for transcriptome analysis is currently missing. Previous attempts to overcome this problem either involved using RNA isolated from

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(Via Nature Protocols.)