Saturday, June 1, 2013

Fwd: MicroRNA target site identification by integrating sequence and binding information

Fwd: please follow footer link

MicroRNA target site identification by integrating sequence and binding information: "

Nature Methods.
doi:10.1038/nmeth.2489

Authors: William H Majoros, Parawee Lekprasert, Neelanjan Mukherjee, Rebecca L Skalsky, David L Corcoran, Bryan R Cullen & Uwe Ohler

High-throughput sequencing has opened numerous possibilities for the identification of regulatory RNA-binding events. Cross-linking and immunoprecipitation of Argonaute proteins can pinpoint a microRNA (miRNA) target site within tens of bases but leaves the identity of the miRNA unresolved. A flexible computational framework, microMUMMIE, integrates sequence with cross-linking features and reliably identifies the miRNA family involved in each binding event. It considerably outperforms sequence-only approaches and quantifies the prevalence of noncanonical binding modes.

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(Via Nature Methods aop.)