Friday, July 12, 2013

Fwd: Chromatin tandem affinity purification sequencing

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Chromatin tandem affinity purification sequencing: "

Nature Protocols 8, 1525 (2013).
doi:10.1038/nprot.2013.088

Authors: Vahab D Soleimani, Gareth A Palidwor, Parameswaran Ramachandran, Theodore J Perkins & Michael A Rudnicki

Chromatin immunoprecipitation coupled with ultra-high-throughput sequencing (ChIP-seq) is a widely used method for mapping the interactions of proteins with DNA. However, the requirements for ChIP-grade antibodies impede wider application of this method, and variations in results can be high owing to differences in affinity and

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(Via Nature Protocols.)