Friday, July 12, 2013

Fwd: De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis

Fwd: please follow footer link

De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis: "

Nature Protocols 8, 1494 (2013).
doi:10.1038/nprot.2013.084

Authors: Brian J Haas, Alexie Papanicolaou, Moran Yassour, Manfred Grabherr, Philip D Blood, Joshua Bowden, Matthew Brian Couger, David Eccles, Bo Li, Matthias Lieber, Matthew D MacManes, Michael Ott, Joshua Orvis, Nathalie Pochet, Francesco Strozzi, Nathan Weeks, Rick Westerman, Thomas William, Colin N Dewey, Robert Henschel, Richard D LeDuc, Nir Friedman & Aviv Regev

De novo assembly of RNA-seq data enables researchers to study transcriptomes without the need for a genome sequence; this approach can be usefully applied, for instance, in research on 'non-model organisms' of ecological and evolutionary importance, cancer samples or the microbiome. In this protocol

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(Via Nature Protocols.)