Monday, September 30, 2013

Fwd: Smart-seq2 for sensitive full-length transcriptome profiling in single cells

Single cell NGS. The dream comes true (does it?)
Fwd: please follow footer link

Smart-seq2 for sensitive full-length transcriptome profiling in single cells: "

Nature Methods.
doi:10.1038/nmeth.2639

Authors: Simone Picelli, Åsa K Björklund, Omid R Faridani, Sven Sagasser, Gösta Winberg & Rickard Sandberg

Single-cell gene expression analyses hold promise for characterizing cellular heterogeneity, but current methods compromise on either the coverage, the sensitivity or the throughput. Here, we introduce Smart-seq2 with improved reverse transcription, template switching and preamplification to increase both yield and length of cDNA libraries generated from individual cells. Smart-seq2 transcriptome libraries have improved detection, coverage, bias and accuracy compared to Smart-seq libraries and are generated with off-the-shelf reagents at lower cost.

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(Via Nature Methods aop.)