Friday, November 29, 2013

Fwd: BSeQC: quality control of bisulfite sequencing experiments

Fwd: please follow footer link

BSeQC: quality control of bisulfite sequencing experiments: "

Motivation: Bisulfite sequencing (BS-seq) has emerged as the gold standard to study genome-wide DNA methylation at single-nucleotide resolution. Quality control (QC) is a critical step in the analysis pipeline to ensure that BS-seq data are of high quality and suitable for subsequent analysis. Although several QC tools are available for next-generation sequencing data, most of them were not designed to handle QC issues specific to BS-seq protocols. Therefore, there is a strong need for a dedicated QC tool to evaluate and remove potential technical biases in BS-seq experiments.

Results: We developed a package named BSeQC to comprehensively evaluate the quality of BS-seq experiments and automatically trim nucleotides with potential technical biases that may result in inaccurate methylation estimation. BSeQC takes standard SAM/BAM files as input and generates bias-free SAM/BAM files for downstream analysis. Evaluation based on real BS-seq data indicates that the use of the bias-free SAM/BAM file substantially improves the quantification of methylation level.

Availability and implementation: BSeQC is freely available at: http://code.google.com/p/bseqc/.

Contact:wl1@bcm.edu

Supplementary information:Supplementary data are available at Bioinformatics online.

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(Via Bioinformatics - current issue.)