Friday, March 14, 2014

Fwd: Qualifying high-throughput immune repertoire sequencing

When NGS meets the Immune Repertoire, 'Close encounter of the Xth kind'?
Fwd: please follow footer link

Qualifying high-throughput immune repertoire se... [Cell Immunol. 2014] - PubMed - NCBI: "Cell Immunol. 2014 Feb 23;288(1-2):31-38. doi: 10.1016/j.cellimm.2014.02.001. [Epub ahead of print]
Qualifying high-throughput immune repertoire sequencing.
Niklas N1, Pröll J2, Weinberger J3, Zopf A4, Wiesinger K5, Krismer K6, Bettelheim P7, Gabriel C8.
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Diversity of B and T cell receptors, achieved by gene recombination and somatic hypermutation, allows the immune system for recognition and targeted reaction against various threats. Next-generation sequencing for assessment of a cell's gene composition and variation makes deep analysis of one individual's immune spectrum feasible. An easy to apply but detailed analysis and visualization strategy is necessary to process all sequences generated. We performed sequencing utilizing the 454 system for CLL and control samples, utilized the IMGT database and applied the presented analysis tools. With the applied protocol, malignant clones are found and characterized, mutational status compared to germline identity is elaborated in detail showing that the CLL mutation status is not as monoclonal as generally thought. On the other hand, this strategy is not solely applicable to the 454 sequencing system but can easily be transferred to any other next-generation sequencing platform."